Fig 5. Flagellin enhanced anti-Ag85B antibody responses following either systemic or mucosal prime-boost immunization.

Mice were primed twice with DNA vaccine via the IM route and boosted either IM or IN with Ad vaccine three weeks later as shown in Table 1. Sera were collected and tested by ELISA for anti-Ag85B IgG antibodies at week 3 post systemic (A) or mucosal (B) boosting. Data shown are means of endpoint antibody titers ± SEM per group. (C) Lungs were harvested from IN-boosted mice at week 14 post-boosting and tested for mucosal IgA-secreting B cells by ELISpot. Data shown are mean of SFCs ± SEM per group. (*p < 0.05; **p < 0.01 vs 85B/85B group). Representative data from one of two independent experiments are shown (n = 5).