Skip to main content
. 2015 Oct 9;6(34):35173–35182. doi: 10.18632/oncotarget.6050

Figure 1. PRMT1 methylates INCENP in vitro.

Figure 1

A. Recombinant INCENP protein was methylated by PRMT1. An in vitro methyltransferase assay was performed by using purified GST-tagged INCENP and PRMT1 recombinant proteins. Methylated INCENP was detected by fluorography. Amounts of loading proteins were evaluated by staining with MemCodeTM Reversible Protein Stain (Thermo Fisher Scientific). B. The LC-MS/MS spectrum corresponding to the mono-methylated INCENP 887–902 peptide of a tryptic digest of which in vitro methylated INCENP by PRMT1. The 14 Da increase of the Arg 887 residue was observed by y15 ion. C. Selected full MS ion chromatograms of unmodified and mono-methylated INCENP 887-902 peptides in the LC-MS/MS. D. The theoretical values of MS/MS fragments ions of the Arg 887 mono-methylated INCENP 887–902 peptides are summarized in the table. The abbrevations of fragment ion types were indicated by the MASCOT program (http://www.matrixscience.com/help/fragmentation_help.html). The observed ions in Figure 1B were indicated in red letters.