Skip to main content
. 2015 Oct 9;6(34):35173–35182. doi: 10.18632/oncotarget.6050

Figure 4. PRMT1 knockdown causes abnormal chromosome alignment and chromosome segregation.

Figure 4

A549 cells A. and HeLa cells B. were transfected with siNC (control) or siPRMT1#1 for 48 h, and then treated with 7.5 μg/ml of aphidicolin for 24 h. Cells were fixed with 4% paraformaldehyde, and then stained with an anti-phospho AURKB antibody (Alexa Fluor® 488, green), an anti-α-Tubulin antibody (Alexa Fluor® 594 [red]) and 4′,6′-diamidine-2′-phenylindole dihydrochloride (DAPI [blue]) 15 h after release of cell cycle. The percentage of cells, which showed abnormal chromosome alignment and chromosome segregation, were 74.4% (A549) and 72.9% (HeLa) to the total number of cells.