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. 2015 Sep 30;6(34):35183–35201. doi: 10.18632/oncotarget.5930

Figure 2. Restoration of miR-145-5p expression restrains in vivo and in vitro tumor invasion.

Figure 2

A. Tumor engraftment of A549 cells subcutaneously injected in 6-weeks old athymic nu/nu mice. B. Human non-small cell lung carcinoma A549-luc and the A549-miR145-5p cells were orthotopically injected below the dura. Tumor cell growth was monitored twice a week conducting quantitative bioluminescence imaging (qBLI) using the IVIS200 imaging station (Caliper Life Sciences), beginning one week after tumor cell injection. C. Numbers of engrafted tumors derived from A549-luc and A549-miR-145-5p cells respectively. D. Bioluminescence images were acquired at day 5, 8 and 20 after the orthotopical injectione with the IVIS Spectrum imaging system (PerkinElmer) and quantified by measurement of photon flux (photons/s/cm2/steradian) using the Living Image Software package (Perkin Elmer/Caliper Life Sciences).