Skip to main content
. 2015 Oct 12;6(34):35304–35314. doi: 10.18632/oncotarget.6095

Figure 3. Macrophages-induced fibrotic responses of fibroblasts are PAR-1 dependent.

Figure 3

A. Wound size of NIH3T3 fibroblast monolayers after treatment with PBS (control) or RAW264.7 conditioned medium (CM) for 18 hours in the presence or absence of TGFBR inhibitors (10 μM SB-431542 and 10 μM LY-2157299) or P1pal-12 (10 μM). Shown are photographs of representative microscopic fields. B. Quantification of the results depicted in A. as described in the Materials and Methods section. Data are expressed as mean±SEM (n = 6 independent experiments). ***P < 0.001. C. Western blot analysis of α-SMA and collagen expression in NIH3T3 cells stimulated with RAW264.7 CM in the absence or presence of P1pal-12 (10 μM) or TGFBR inhibitors (10 μM of SB-431542 and 10 μM of LY-2157299). Tubulin served as loading control. Gels have been run under the same experimental conditions and uncropped blots are shown in Supplementary Figure.

HHS Vulnerability Disclosure