Figure 4. Common Upstream regulators determined by Ingenuity Pathway Analysis (IPA) software from the significant genes for old mice compared to all other age groups for tumor bearing and tumor non-bearing mice.

Gene network depiction of the common upstream regulators for old mice compared to all other age groups predicted to be either activated (orange) or inhibited (blue) determined by IPA software. Specific upregulated (red) and downregulated (green) genes from the experimental data set involved in determining the activation state of the upstream regulator are shown with direct (solid lines) and indirect (dashed lines) relationships to the upstream regulators. A predicted relationship is color coded to indicate whether it leads to activation (orange) or inhibition (blue). Relationships that are inconsistent with the prediction (yellow) or have an undetermined effect (grey) are also shown. The darker the shade of green or red, the greater the fold change. Below each network is a schematic of the activation states of the upstream regulators from Table 1 illustrating the balance between the tumor promoters (text in yellow) and tumor suppressors (text in white and underlined) with a predicted activation (circled in orange) or predicted inhibition (circled in blue). Upstream regulators that are predicted to have both tumor promoting and suppressing characteristics are shown with black text.