Figure 6. Key immune molecular factors in spleen related to key molecule affecting tumor progression as a function of age.

A. The mRNA expression determined by real-Time PCR (RTPCR) for CCL19, CD2, CCL5, and CD3E and quantified protein expression from western blots for CD2 and CD3E compared to the gene expression observed by Illumina. Log₂ fold changes for mRNA and protein expression were determined for each group. * p < 0.05. B. Representative western blots for CD2 and CD3e with technical control GAPDH for Adolescent (A), Young Adult (Y), Middle-Aged (M), and Old (O) mice C. Pathway analysis was done with Ingenuity Pathway Analysis (IPA) software. INFG was added to the network to provide relations and connections between all genes to TGFβ1. Predicted activity due to other genes is also shown for INFG. D. A Protein-Protein Interaction (PPI) network was constructed using STRING for the key genes including the relation to TGFβ1 through IFNG. The nodes are connected through lines indicating the relationship between each protein. The color of the lines indicates the evidence of the connection between each protein. The protein structure is shown within the node.