Table 3. Primer sequences used for multiplex PCR.
PCR combination | Locus | Primer+ | Primer− | Fluorescent modification |
---|---|---|---|---|
Multiplex PCR in Group1 | G5004 | GGTCAAACCTTTCGTCAAT | TTTTATGCAGGAACTCTTACTAC | 5′ROX |
G5006 | ATTCTGCTTTACTTTATGACACG | AGGTTTTATTTTGCCATACATTT | 5′ROX | |
G5010 | CATTTTACTGCTTGCCTCAC | CCCTTCCTTTCGCATAGA | 5′ROX | |
Multiplex PCR in Group 2 | G5011 | AAGCCACCAACCTCTACGA | TAACAGGGATGGGATGAAAT | 5′TAMRA |
G5023 | CCAAGACCCAGTGGAAAC | CTCTACTGGCCTCTAGTGTGA | 5′FAM | |
G5024 | ATTCCTTCCGAAATCAGTG | AGAGGGAGAAAGATAAGACCA | 5′HEX | |
Multiplex PCR in Group 3 | G5020 | CAACCCTGTTTCTGTCCTGT | GCAAGCAACTGTCAACCTG | 5′FAM |
G5036 | ACTTTTTGTTAACCTAAACCTCT' | ACACACCCTCACATACACTCTC | 5′ROX |