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. 2015 Oct 2;6(34):35667–35683. doi: 10.18632/oncotarget.5523

Figure 1. Effect of ABT-737 on cell viability and apoptosis in human oral cancer cells.

Figure 1

MC-3 and HN22 cells were treated with or without ABT-737 for 24 hr at different concentrations. Cell viability was evaluated using a MTS assay A. and trypan blue dye exclusion assay B. Qualitative estimation of ABT-737-induced apoptotic cell death was obtained by a live/dead assay, which differentially labels live (green) and dead (red) cells with fluorescent dyes C. With fluorescence microscopy (magnification X200), MC-3 and HN22 cells exhibited nuclear fragmentation and condensation, as determined by DAPI staining D. The results are presented as the mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001.