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. 2015 Oct 2;6(34):35667–35683. doi: 10.18632/oncotarget.5523

Figure 2. Effect of ABT-737 on caspase-mediated apoptosis in human oral cancer cells.

Figure 2

MC-3 and HN22 cells were treated with or without ABT-737, and the expression levels of cleaved caspase-9 and 3 and cleaved PARP were analyzed by Western blot analysis A. MC-3 and HN22 cells were preincubated with a pancaspase inhibitor (Z-VAD-FMK) for 1 hr prior to treatment with ABT-737; afterwards, cells were harvested for Western blot analysis to detect cleaved caspase-3 and cleaved PARP B. Immunocytochemistry revealed the release of mitochondrial cytochrome c (green) into the cytoplasm after ABT-737 exposure (20 μM in MC-3 cells and 22.5 μM in HN22 cells) for 24 hr. MitoTracker (red) staining labels mitochondria within each field C. β-actin was used as an internal control. The results are shown as the mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001.