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. 2015 Sep 30;6(34):35737–35754. doi: 10.18632/oncotarget.5927

Figure 1. Endogenous SPINK1 expression drives proliferation of ovarian cancer cells.

Figure 1

A. SPINK1 transcripts were measured in ovarian cancer cell lines by qRT/PCR normalizing to GAPDH; values on y-axis show expression relative to OVCAR3. B. UWB1.289 cells transduced with shRNA lentiviruses KD1 and KD2 targeting SPINK1 show effective knockdown relative to cells transduced with non-target control virus (NT), as assessed by qRT/PCR (KD1 p = 0.0005, KD2 p = 0.0001). C. SPINK1 knockdown in UWB1.289 cells results in significant reduction in metabolically active cells as assessed by MTT assay (KD1 p = 0.0527, KD2 p = 0.0115). D. OVCA420 cells transduced with shRNA lentiviruses KD1 and KD2 targeting SPINK1 show effective knockdown relative to cells transduced with non-target control virus (NT), as assessed by qRT/PCR (KD1 p = 0.0228, KD2 p = 0.0258). E. SPINK1 knock-down in OVCA420 cells shows significant reduction in metabolically active cells as assessed by MTT assay (KD1 p = 0.0001, KD2 p = 0.0001). F. SPINK1 knockdown in UWB1.289 cells results in significantly reduced proliferation in EdU assay (KD1 p = 0.0002, KD2 p = 0.0001). G. SPINK1 knockdown in OVCA420 cells results in significantly reduced proliferation in EdU assay (KD1 p = 0.002, KD2 p = 0.0013). *p < 0.05; **p < 0.01; ***p < 0.0001 (unpaired t-test).