Skip to main content
PMC home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1969 Jul;22(4):470–474. doi: 10.1136/jcp.22.4.470

Sensitivity to amphotericin B of a Naegleria sp. isolated from a case of primary amoebic meningoencephalitis

Rodney F Carter 1
PMCID: PMC474214  PMID: 5798635

Abstract

An amoeba of the genus Naegleria causing fatal meningoencephalitis in a human subject has been investigated for its sensitivity to several drugs. Penicillin, sulphadiazine, chloramphenicol, oxytetracycline hydrochloride, streptomycin, methotrexate, emetine, quinine, and metronidazole had no effect on the organism in vitro at levels in excess of those likely to be attained therapeutically in the brain. Amphotericin B was highly amoebicidal in vitro and protected mice from infection with the organism. Used in high dosage by the intraventricular as well as the intravenous route, this drug might be successful in the treatment of further cases of primary amoebic meningoencephalitis.

Full text

PDF
473

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDRIOLE V. T., KRAVETZ H. M. The use of amphotericin B in man. JAMA. 1962 Apr 28;180:269–272. doi: 10.1001/jama.1962.03050170001001. [DOI] [PubMed] [Google Scholar]
  2. BALAMUTH W. Comparative action of selected amebicidal agents and antibiotics against several species of human intestinal amebae. Am J Trop Med Hyg. 1953 Mar;2(2):191–205. doi: 10.4269/ajtmh.1953.2.191. [DOI] [PubMed] [Google Scholar]
  3. BAND R. N. Nutritional and related biological studies on the free-living soil amoeba, Hartmannella rhysodes. J Gen Microbiol. 1959 Aug;21:80–95. doi: 10.1099/00221287-21-1-80. [DOI] [PubMed] [Google Scholar]
  4. Butt C. G. Primary amebic meningoencephalitis. N Engl J Med. 1966 Jun 30;274(26):1473–1476. doi: 10.1056/NEJM196606302742605. [DOI] [PubMed] [Google Scholar]
  5. CRAMBLETT H. G. Recognition and treatment of intracranial manifestations of leukemia. AMA J Dis Child. 1959 Jun;97(6):805–812. doi: 10.1001/archpedi.1959.02070010807007. [DOI] [PubMed] [Google Scholar]
  6. Callicott J. H., Jr Amebic meningoencephalitis due to free-living amebas of the Hartmannella (Acanthamoeba)-Naegleria group. Am J Clin Pathol. 1968 Jan;49(1):84–91. doi: 10.1093/ajcp/49.1.84. [DOI] [PubMed] [Google Scholar]
  7. Carter R. F. Primary amoebic meningo-encephalitis: clinical, pathological and epidemiological features of six fatal cases. J Pathol Bacteriol. 1968 Jul;96(1):1–25. doi: 10.1002/path.1700960102. [DOI] [PubMed] [Google Scholar]
  8. Cerva L., Novăk K. Amoebic meningoencephalitis: 16 fatalities. Science. 1968 Apr 5;160(3823):92–92. doi: 10.1126/science.160.3823.92. [DOI] [PubMed] [Google Scholar]
  9. Culbertson C. G., Ensminger P. W., Overton W. M. Pathogenic Naegleria sp.--study of a strain isolated from human cerebrospinal fluid. J Protozool. 1968 May;15(2):353–363. doi: 10.1111/j.1550-7408.1968.tb02136.x. [DOI] [PubMed] [Google Scholar]
  10. DONOVICK R., GOLD W., PAGANO J. F., STOUT H. A. Amphotericins A and B, antifungal antibiotics produced by a streptomycete. I. In vitro studies. Antibiot Annu. 1955;3:579–586. [PubMed] [Google Scholar]
  11. Davies A. H. Metronidazole in human infections with syphilis. Br J Vener Dis. 1967 Sep;43(3):197–200. doi: 10.1136/sti.43.3.197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Di Chiro G. Observations on the circulation of the cerebrospinal fluid. Acta Radiol Diagn (Stockh) 1966;5:988–1002. doi: 10.1177/02841851660050p242. [DOI] [PubMed] [Google Scholar]
  13. Fowler M., Carter R. F. Acute pyogenic meningitis probably due to Acanthamoeba sp.: a preliminary report. Br Med J. 1965 Sep 25;2(5464):740–742. doi: 10.1136/bmj.2.5464.734-a. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. KRESS M. B., CANTRELL J. R. PULMONARY AND MENINGEAL CRYPTOCOCCOSIS: SUCCESSFUL TREATMENT OF THE MENINGITIS WITH LATERAL CEREBRAL INTRAVENTRICULAR INJECTION OF AMPHOTERICIN B. Arch Intern Med. 1963 Sep;112:386–392. doi: 10.1001/archinte.1963.03860030140015. [DOI] [PubMed] [Google Scholar]
  15. LENSHOEK C. H., BAUMANN C., WIELENGA D. K. Brain abscess due to Entamoeba histolytica; case report with successful excision. Arch Chir Neerl. 1958;10(1):34–40. [PubMed] [Google Scholar]
  16. OURA M., STERNBERG T. H., WRIGHT E. T. A new antifungal antibiotic, amphotericin B. Antibiot Annu. 1955;3:566–573. [PubMed] [Google Scholar]
  17. PRATA A. TREATMENT OF KALA-AZAR WITH AMPHOTERICIN B. Trans R Soc Trop Med Hyg. 1963 Jul;57:266–268. doi: 10.1016/0035-9203(63)90183-4. [DOI] [PubMed] [Google Scholar]
  18. Patras D., Andujar J. J. Meningoencephalitis due to Hartmannella (Acanthamoeba). Am J Clin Pathol. 1966 Aug;46(2):226–233. [PubMed] [Google Scholar]
  19. Powell S. J., MacLeod I., Wilmot A. J., Elsdon-Dew R. Metronidazole in amoebic dysentery and amoebic liver abscess. Lancet. 1966 Dec 17;2(7477):1329–1331. doi: 10.1016/s0140-6736(66)92081-2. [DOI] [PubMed] [Google Scholar]
  20. RIESELBACH R. E., DI CHIRO G., FREIREICH E. J., RALL D. P. Subarachnoid distribution of drugs after lumbar injection. N Engl J Med. 1962 Dec 20;267:1273–1278. doi: 10.1056/NEJM196212202672502. [DOI] [PubMed] [Google Scholar]
  21. TAYLOR R. L., LYNCH H. P., TAYLOR R. R., WEISER O. L. The determination of serum concentrations of amphotericin B in man. Am Rev Tuberc. 1958 Jun;77(6):1023–1025. doi: 10.1164/artpd.1958.77.6.1023. [DOI] [PubMed] [Google Scholar]
  22. WINN W. A. COCCIDIOIDOMYCOSIS AND AMPHOTERICIN B. Med Clin North Am. 1963 Sep;47:1131–1148. doi: 10.1016/s0025-7125(16)33522-2. [DOI] [PubMed] [Google Scholar]
  23. Witorsch P., Williams T. W., Jr, Ommaya A. K., Utz J. P. Intraventricular administration of amphotericin B. Use of subcutaneous reservoir in four patients with mycotic meningitis. JAMA. 1965 Nov 15;194(7):699–702. doi: 10.1001/jama.194.7.699. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES