Figure 5. Blockage of integrin αvβ3 activity by antibody inhibits DHT-induced PI3K activation in resveratrol-treated MDA-MB-231 cells.
MDA-MB-231 cells were pre-treated with anti-integrin αvβ3 antibody for 24 h prior to treatment of 10−9 M DHT or 10 μM resveratrol for another 24 h. The cells were stained for anti-pSer15-p53 antibody or anti-phosphoPI3K (pPI3K p85) antibody, and Hoechst stain for nucleus. These merged images were presented by using confocal microscopy. Treatment of resveratrol induced nuclear translocation of pSer15-p53, but this action was blocked when integrin αvβ3 was neutralized. The expressions of pPI3K were induced by DHT in cytosol, but were attenuated by integrin αvβ3 neutralization. The scale bar was presented on the right bottom of each image.