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. 2015 Oct 3;6(34):35978–35990. doi: 10.18632/oncotarget.5427

Figure 2. Effect of miR-573 on cell motility in vitro and and tumor metastasis in vivo.

Figure 2

Transwell migration A, B. and invasion C, D. assays were applied to detect the invasive and migratory potentials of VCaP and PC3 cells after transfection with the miR-573 mimics (left), its antagomir (right) or their controls, respectively (200 nM). E. VCaP stably transfected with miR573 and its controls were injected subcutaneously into the flanks of nude mice (n = 10). The metastatic foci in the lung is shown in (E) and quantified in F. G. Representative H&E staining images of lungs from one mouse per treatment group are shown. miR-573 mimics transfection showed significantly reduced number of metastatic lesions (red arrow). H. Tumor volumes of tumor mouse model were measured. I. Western blot was performed to detect the expression of E-cadherin, Vimentin and Snail in VCaP and 22RV1 cells during TGF-β1 induced EMT. Mimics/antagomir control: cells transfected with the mimics/antagomir control; miR-573 mimics/antagomir: cells transfected with the miR-573 mimics/antagomir. *p < 0.05, **p < 0.01. Data in F are means of biological triplicates (± standard error) and are representative of duplicate (E–H) or triplicate (A–D) experiments.