A., Establishment of PANC-1 cell lines stably expressing vector, YAP, YAP3D, YAP-S127A, and YAP4A (S127A/3A). 4A: S127A/T119A/S289A/S367A; 3D:T119D/S289D/S367D. B.-E., Cell migration (B and D) and invasion (C and E) assays with PANC-1 cells expressing various YAP constructs. F., Establishment of BxPC3 cell lines stably expressing vector, YAP, YAP3D, YAP-S127A, and YAP4A (S127A/3A). 4A: S127A/T119A/S289A/S367A; 3D:T119D/S289D/S367D. G.-J., Cell migration (G and I) and invasion (H and J) assays with BxPC3 cells expressing various YAP constructs. Cell migration assays with Transwell and invasion assays with Matrigel were performed as we have previously described [14]. Migrated and invaded cells were stained with DAPI, and representative fields are shown. Data are expressed as the mean ± s.e.m. of three experiments. ***: p < 0.001, **: p < 0.01 (t-test).