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. 2015 Sep 25;6(34):36156–36171. doi: 10.18632/oncotarget.5392

Figure 4. MDM2 expression affects migration and docetaxel sensitivity of prostate epithelial cells.

Figure 4

A. BPH-1 cells were transiently transfected with control or MDM2-specific siRNA and cell migration was evaluated by monitoring the impedance signal using a fibronectin-coated CIM module on an xCELLigence DP system. The graph shows the average Cell Index values (mean ± SD) from three independent experiments, *P < 0.05. B. Migration results through an uncoated 8 μm-pore transwell from three independent BPH-1 clones stably transfected with control or MDM2-specific shRNA. C, D. Transwell migration of a pool of CAFTD03 cells transiently overexpressing (C) wt, GFP-tagged or the C464A mutant of MDM2 or (D) MDMX. Graphs represent the relative numbers of migrating cells (mean ± SD) from 3 independent experiments in technical duplicate; *P < 0.05; n.s., P > 0.05. EV, empty vector. E. Viability of stable shRNA-expressing clones from BPH-1 cells treated with docetaxel for 72 h was analyzed using a luminescence-based ATP assay. Graphs show the mean ± SD from 3 independent experiments; IC50 values were calculated from 3 independent experiments.