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. 2015 Sep 22;6(34):36383–36399. doi: 10.18632/oncotarget.5799

Figure 5. SP alters microtubule architecture in a dose-dependent manner.

Figure 5

The effect of SP on tubulin polymerization and acetylation was analyzed. A. Representative images of western blots of polymeric and monomeric tubulin fractions and their acetylation levels at Lys 40 following treatment with 10 μM SP up to 72 hours. B. Quantification of polymeric and monomeric tubulin acetylation levels. C. Quantification of the relative amounts of tubulin fractions. D. Confocal images of whole cell acquisitions (left columns) and relative 4X magnification details (right columns) showing acetylated microtubules architecture after DMSO (CTRL), 10 μM (SP10) and 20 μM (SP20) SP incubation for 72 hours. Acetylated microtubule network, green staining; nuclei labeled with DAPI, blue staining; scalebars: 30 μm. *p < 0.05, **p < 0.01, ***p < 0.001 vs day 0.