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. 2015 Sep 28;6(34):36505–36521. doi: 10.18632/oncotarget.5364

Figure 5. Treatment with AF induces ER stress in human gastric cancer cells.

Figure 5

A–B. BGC-823 A. or SGC-7901 B. cells were treated with AF (4 μM) for the indicated times, the protein levels of p-PERK, p-eIF2α, ATF4 and CHOP were determined by western blot. C–D. SGC-7901 cells transfected with CHOP siRNA or control siRNA were treated with AF (4 μM) for 24 h. Percentage of cell apoptosis was determined by Annexin-V/PI staining and flow cytometry. E–F. NAC totally reversed the activation of ER stress induced by AF. BGC-823 or SGC-7901 cells were pretreated with or without 5 mM NAC for 2 h before exposure to AF, three hours later the p-eIF2α and ATF-4 expression were detected by western blot. The protein level of CHOP was examined by western blot after treatment with AF for 12 h. GAPDH was used as internal control. Data presented are representative of three independent experiments.