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. 2015 Sep 28;6(34):36505–36521. doi: 10.18632/oncotarget.5364

Figure 6. AF activates mitochondrial apoptotic pathway.

Figure 6

A. Treatment of cells with AF obviously decreased the mitochondrial membrane potential (Δψm). SGC-7901 cells after treatment AF (4 μM) for 14 h were stained with JC-1 and then analyzed by fluorescence microscope. B–C. Caspase-9 activity. Cells after treatment with AF for 20 h were lysed and cell proteins were used to determine caspase-9 activity with assay kit using specific substrate. D. Treatment with AF provoked the release of cytochrome C from mitochondria into cytosol. SGC-7901 cells after treatment AF (4 μM) for 14 h were stained with Mito-T green fluorescence, anti-cytochrome C and DAPI for detecting the translocation of cytochrome C from the mitochondria to the cytosol. E. Western blot analysis effects of AF on expression of Bcl-2 family proteins in SGC-7901 cells. A–E. NAC significantly reversed the activation of mitochondrial apoptotic pathway induced by AF. All images shown here are representative of three independent experiments with similar results.