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. 2015 Oct 2;6(34):36762–36773. doi: 10.18632/oncotarget.5461

Figure 3. Characterization of mechanisms underpinning CD44-promoted integrin activation.

Figure 3

A. A panel of immunoblots characterizing siRNA-mediated knockdown of Talin (TLN), CD44 or RHAMM expression, and their subsequent effect on HA-induced activation of the β1-integrin receptor pool in MDA-MB-231 cells. Cells were stimulated with HA for 2 mins to focus understanding of the early events occurring downstream of CD44 activation. B. Immunoblots presenting the analysis of protein lysates harvested by co-immunoprecipitation experiments using anti-CD44 or anti-IgG antibodies (control). Cells were again stimulated with HA for 2 min to characterize the molecular interactions induced immediately downstream of CD44 initiated inside-out signalling. C. Representative blots of co-immunoprecipitation experiments characterizing the complex of CD44 with β1-integrins under HA stimulated (20 min) and talin-depleted conditions. Blots shown are representative of three independent experiments. The experimental design focused on understanding the importance of talin depletion on downstream integrin activation, thus introducing a longer incuation time. Immunoblots shown throughout the Figure are representative of three or more independent experiments.