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. 2015 Sep 15;6(34):36789–36814. doi: 10.18632/oncotarget.5103

Figure 8. Crosstalk of Shh and Bcl3 signaling pathways in BCC cells regulate proliferation.

Figure 8

A. mRNA expression of N-cadherin and cyclin D1 in skin of UVB-irradiated and Shh-inhibitors-treated. B. Percentage of ASZ001 cells showing nuclear localization of Bcl3 in GANT61 (10 μm) and Cyclopamine (5 μm)-treated cells. C. TaqMan real-time PCR showing effects of Isopropyl β-D-1-thiogalactopyranoside (IPTG) treatment on the expression of Bcl3, CyclinD1 and N-cadherin in ASZ001 cells. ASZ001 cells have been infected with IPTG-inducible Bcl3 shRNA lentiviral vector and were selected following treatment with puromycin containing growth medium. D. TaqMan real-time PCR showing the expression of shh signaling related genes Gli1, Gli2 and Ptch1 following knockdown of Bcl3 in ASZ001 cells. E. Colony formation assay in control, IPTG (1 mM), Cyclopamine (5 μm) and IPTG+Cyclopamine treated ASZ001 cells. F. Bar diagram showing colony numbers following various treatments.