Fig 2. Immunostaining of Kv7 channels in the CA.
A, 10 μM ML277, an opener of Kv7.1 channels, did not affect the tension in histamine-pre-contracted domestic pig CA rings. A sample trace is shown. B and C, Shown are sample isometric tension recordings illustrating that a Kv7.4 activator diclofenac (200 μM) causes greater dilations of histamine-pre-contracted rings than a Kv7.5 activator UCL2077 (50 μM). D, Summary data for “A-C” (ML213: n = 12; Diclofenac: n = 5; ML277, n = 8; UCL2077: n = 5). In these experiments, we selected those domestic pig CA rings that exhibited the slower decay rate of histamine-induced contractions. E and F, Images illustrating typical immunohistochemical staining patterns for Kv7.4 and Kv7.5 isoforms in the domestic pig CA wall.