Fig 1. Survival rates and virus titers in IFN-α/β/γR KO mice infected with DV3P12/08.

(A) Groups of IFN-α/β/γR KO mice (5–6 weeks old) were intraperitoneally infected with DV3P12/08 at doses ranging from 2 × 10⁶ to 2 × 10² focus-forming units (FFU) or (B) subcutaneously infected with 2.6 × 10⁶ FFU of DV3P12/08 and survival monitored. (C) Groups of IFN-α/βR KO mice (either 5 weeks old or 11 weeks old) were intraperitoneally infected with 1.3 × 10⁷ FFU of DV3P12/08 and survival was monitored. (D) IFN-α/β/γR KO mice (5–6 weeks old) were intraperitoneally infected with 2 × 10⁶ of DV3P12/08, sacrificed at Day 5 p.i. under anesthesia, and perfused extensively with PBS. Virus copy numbers in the spleen, liver, kidney, thymus, lung, brain, peritoneal exudate cells (PEC), bone marrow (BM), upper (U) small intestine, lower (L) small intestine, large intestine, and serum were then measured by qRT-PCR. Bars indicate the mean value. Each symbol represents an individual mouse.