Fig 2. IFN-α/β/γR KO mice infected with DV3P12/08 suffer vascular leakage.

(A) Mice (5–6 weeks old) were intraperitoneally infected with 2 × 10⁶ focus-forming units (FFU) of DV3P12/08 or PBS (mock-infected). Moribund mice were intravenously injected with Evans blue dye on Day 5 p.i., sacrificed under anesthesia, and perfused extensively with PBS. Extravasation of the dye into the peritoneal cavity, (B) intestines, (C) brain, (D) kidneys, and (E) liver of mock-infected or DV3P12/08-infected mice. (F) Quantification of Evans blue in the spleen, liver, kidneys, lungs, brain, upper small intestine, lower small intestine, and large intestine of infected and mock-infected mice (n = 6/group). *P < 0.05 and **P < 0.01.