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. 2016 Feb 4;11(2):e0148564. doi: 10.1371/journal.pone.0148564

Fig 5. TNF-α plays a critical role in lethal DV3 12/08 infection.

Fig 5

(A) Survival of IFN-α/β/γR knockout (KO) mice intraperitoneally infected with 2 × 106 focus-forming units (FFU) of DV3P12/08, followed by the intraperitoneal injection with an anti-TNF-α Ab (n = 6) or an isotype control Ab (n = 6) on Days 1, 2, 4 p.i. Survival differences were statistically significant between the anti-TNF-α-treated group and the isotype control (p = 0.0004), the anti-TNF-α-treated group and the anti-IL6-treated group (p = 0.0023), the anti-IL6-treated group and the isotype control (p = 0.0036). (B) Sera were collected from anti-TNF-α Ab- (n = 6) or isotype control Ab- (n = 5) treated mice on Days 5 and 6 p.i. and the viral copy number determined by qRT-PCR. (C) Sera were collected from mock- (n = 3) or DV3P12/08 (2 × 106 FFU) (n = 6) infected mice and the levels of TNF-α, IL-6, MCP-1, IFN-γ were measured by qRT-PCR. Bars indicate the mean values. Each symbol represents an individual mouse.