Fig 6. Induced expression of BS69CC-MYND in LCLs leads to a strong debilitation of cell growth.
(A) The retroviral vector mediated inducible expression vector of DD-BS69CC-MYND wild type, Q546A, or Q546A/W550A was used to transduce two LCLs, LCL#1 and LCL#2, and BJAB control cell lines. DD-BS69: DD-BS69 wild-type; DD-Q546: DD-BS69 Q546A; DD-BS69 Q/W: DD-BS69 Q546A/W550A (B) 105 per mL of the above retrovirus transduced cell lines were aliquoted into 6-well plates and cell viability assays were conducted with the treatment of inducer (shield 1) or PBS (negative control) every 24 hrs for five consecutive days. The expression of actin was used internal control.