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. Author manuscript; available in PMC: 2017 Feb 15.
Published in final edited form as: Mol Cell Endocrinol. 2015 Dec 13;422:160–171. doi: 10.1016/j.mce.2015.11.033

Fig. 1.

Fig. 1

4-OHT-induced extracellular factor inhibits IGF-1-stimulated AKT phosphorylation. Conditioned medium (CM) from vehicle-treated MCF-7 cells (24 hours) was replaced with conditioned medium from 4-OHT-treated cells prior to IGF-1 stimulation (50 ng/mL) for 15 min. Total AKT and phospho-AKT were measured by immunoblot. *, p<0.05 compared to vehicle (V) control, and #, p<0.05 compared to IGF-1 treated sample. The results are representative of three independent experiments, and the error bars are SEM.