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. Author manuscript; available in PMC: 2017 Feb 15.
Published in final edited form as: Mol Cell Endocrinol. 2015 Dec 13;422:160–171. doi: 10.1016/j.mce.2015.11.033

Fig. 3.

Fig. 3

CREB was phosphorylated after treatment with 4-OHT and CREB knockdown partially inhibited 4-OHT-induced IGFBP-1 transcription in MCF-7 cells. (A) Immunoblot analysis of phospho-CREB (S133) after 2 hours of treatment with 4-OHT using the indicated doses. (B) Immunoblot analysis of CREB expression after transfection of CREB-targeting siRNA. (C) Relative IGFBP-1 gene transcription determined by quantitative real-time PCR in CREB knockdown and control cells (NT) after 24 hours of treatment with 4-OHT. *, p<0.05 compared to control, and #, p<0.05 compared to 4-OHT (1μM) treated sample. The results are representative of three independent experiments, and the error bars are SEM.