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. Author manuscript; available in PMC: 2017 Feb 15.
Published in final edited form as: Mol Cell Endocrinol. 2015 Dec 13;422:160–171. doi: 10.1016/j.mce.2015.11.033

Fig. 4.

Fig. 4

GPER1-mediated CREB phosphorylation in MCF-7 cells. (A, B) The total ERα and GPER1 protein levels were reduced using siRNA knockdown. (C) Immunoblot analysis of CREB phosphorylation after 2 hours of treatment with 1μM 4-OHT subsequent to ERα or GPER1 knockdown compared to non-targeting siRNA (NT) cells. *, p<0.05 compared to control. The results are representative of three independent experiments, and the error bars are SEM.