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. Author manuscript; available in PMC: 2017 Feb 15.
Published in final edited form as: Mol Cell Endocrinol. 2015 Dec 13;422:160–171. doi: 10.1016/j.mce.2015.11.033

Fig. 5.

Fig. 5

4-OHT-induced IGFBP-1 transcription and inhibition of IGF-1 stimulation is decreased after GPER1 knockdown in MCF-7 cells. (A) Relative IGFBP-1 gene transcription determined by quantitative real-time PCR in GPER1 knockdown and non-targeting control cells (NT) after 24 hours of treatment with vehicle or 1μM 4-OHT. (B) Immunoblot analysis p-IGF-1R, p-AKT and p-ERK1/2 after IGF-1 stimulation in control and GPER1 knockdown MCF-7 cells. (C) MCF-7 cell viability after treatment with 1μM 4-OHT with and without 1μM G36 treatment. *, p<0.05 compared to vehicle and #, p<0.05 compared to G36- treated sample. The results are representative of three independent experiments, and the error bars are SEM.