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(A) Structures of the engineered polyketide 2-propargylerythromycin, resulting from the selection of 2-propargylmalonate by AT5DEBS, and the pre-monensin series, derived from the feeding a variety of “non-native” precursors for generation of novel derivatives. (B) The two distinct routes established for the provision of fluoromalonyl-CoA (58) and the suite of fluorinated polyketide molecules generated from the minimal DEBS system.
