. Author manuscript; available in PMC: 2016 Oct 12.

Published in final edited form as: Chembiochem. 2015 Sep 1;16(15):2191–2199. doi: 10.1002/cbic.201500308

Table 1. Δ6-protoilludene synthases achieve maximum velocity at different concentrations of divalent metal ions.

Concentration of activating metal ion (mM) that achieved V_max with a fixed concentration of (2E,6E)-FPP (100 µM) in a coupled spectrophotometric assay is defined as [A]_max^[36].

	[A]_max (mM)

	Mg²⁺	Ca²⁺	Mn²⁺	Ni²⁺	Co²⁺
Stehi25180	0.10 ± 0.02	0.99 ± 0.14	0.28 ± 0.05	0.38 ± 0.02	0.38 ± 0.03
Stehi64702	0.10 ± 0.07	0.81 ± 0.09	0.10 ± 0.03	0.48 ± 0.06	0.49 ± 0.05
Stehi73029	0.16 ± 0.01	0.56 ± 0.07	0.21 ± 0.03	0.34 ± 0.05	0.36 ± 0.04
Omp6	0.11 ± 0.09	1.44 ± 0.18	0.29 ± 0.06	0.53 ± 0.09	0.62 ± 0.09
Omp7	0.12 ± 0.01	1.81 ± 0.22	0.17 ± 0.01	0.44 ± 0.07	0.46 ± 0.07