Table 2. Metal selectivity mediates product specificity in Stehi73029.
Varying ratios of divalent metal ions (M2+) results in varying levels of 1,11 and 1,10 cyclization products. Amount of each product detected by GC-MS is represented as a percentage of the total abundance of sesquiterpenes produced, calculated from peak areas.
| M2+ | [M2+:M2+] (mM) |
Level of sesquiterpene products (%) | Ratio 1,11:1,10 |
||||||
|---|---|---|---|---|---|---|---|---|---|
| (1) | (2) | (3) | (4) | (5) | (6) | ||||
| Δ6-protoilludene | β-elemene | (E)-β-caryophyllene | 4,11-selinadiene | β-selinene | α-selinene | ||||
| Mg | 10:0 | 100 | 0 | 0 | 0 | 0 | 0 | 100 | 0 |
| Mn | 10:0 | 89 | 6.5 | 4.5 | 0 | 0 | 0 | 93.5 | 6.5 |
| Ca | 10:0 | 21.7 | 68.2 | 5.7 | 0 | 2.3 | 2.1 | 27.4 | 72.6 |
| Mg:Mn | 10:10 | 80.1 | 0 | 19.9 | 0 | 0 | 0 | 100 | 0 |
| Mg:Mn | 10:5 | 100 | 0 | 0 | 0 | 0 | 0 | 100 | 0 |
| Mg:Mn | 10:1 | 100 | 0 | 0 | 0 | 0 | 0 | 100 | 0 |
| Mg:Mn | 5:10 | 100 | 0 | 0 | 0 | 0 | 0 | 100 | 0 |
| Mg:Mn | 1:10 | 72.9 | 9.6 | 17.5 | 0 | 0 | 0 | 90.4 | 9.6 |
| Mg:Ca | 10:10 | 100 | 0 | 0 | 0 | 0 | 0 | 100 | 0 |
| Mg:Ca | 10:5 | 100 | 0 | 0 | 0 | 0 | 0 | 100 | 0 |
| Mg:Ca | 10:1 | 100 | 0 | 0 | 0 | 0 | 0 | 100 | 0 |
| Mg:Ca | 5:10 | 97.9 | 0 | 2.1 | 0 | 0 | 0 | 100 | 0 |
| Mg:Ca | 1:10 | 67.9 | 32.1 | 0 | 0 | 0 | 0 | 67.9 | 32.1 |
| Mn:Ca | 10:10 | 75.2 | 12.9 | 11.9 | 0 | 0 | 0 | 87.1 | 12.9 |
| Mn:Ca | 10:5 | 80.3 | 9.3 | 10.4 | 0 | 0 | 0 | 90.7 | 9.3 |
| Mn:Ca | 10:1 | 86.9 | 4.5 | 8.6 | 0 | 0 | 0 | 95.5 | 4.5 |
| Mn:Ca | 5:10 | 72.8 | 7.4 | 11.3 | 0 | 8.5 | 0 | 84.1 | 15.9 |
| Mn:Ca | 1:10 | 60.8 | 11.9 | 15.3 | 0 | 12 | 0 | 76.1 | 23.9 |