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. 2016 Feb 5;9:9. doi: 10.3389/fnmol.2016.00009

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Copyright © 2016 Ma and Xue.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IDV ratio of IS and S in occludin and claudin-5 as detected by Western blot after overexpression or silencing of miR-200b. Soluble fragments (S) of occludin and claudin-5 represent dephosphorylated forms and are susceptible to non-ionic detergent extraction. Insoluble fragments (IS) of occludin and claudin-5 represent phosphorylated forms, which combine with actin microfilaments through zonula occludens (ZOs) and are resistant to non-ionic detergent extraction. Values are means ± SD (n = 5/group). ^*P < 0.05 vs. control group; ^#P < 0.05 vs. pre-miR-con group; ^▵P < 0.05 vs. anti-miR-con group; ^▿P < 0.05 vs. pre-miR-200b group.