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. 2016 Feb 5;9:9. doi: 10.3389/fnmol.2016.00009

Figure 8.

Figure 8

Effect of RMP7 on expression of RhoA and ROCKII in GECs of the BTB. GECs from RMP7 groups were treated with RMP7 (5 nmol/l) for 0, 5, 10, 15, 30, or 60 min. (A) Relative mRNA expressions of RhoA and ROCKII as determined by quantitative RT-PCR. GAPDH RNA levels were used as an endogenous control. Values are means ± SD (n = 5/group). *P < 0.05 vs. control group; #P < 0.05 vs. RMP7 0 min group;P < 0.05 vs. RMP7 15 min. (B,C) Protein expressions of RhoA and ROCKII as assessed with Western blot. Values are means ± SD (n = 5/group). *P < 0.05 vs. control group; #P < 0.05 vs. RMP7 0 min group;P < 0.05 vs. RMP7 15 min.