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. 2016 Feb 5;9:9. doi: 10.3389/fnmol.2016.00009

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Copyright © 2016 Ma and Xue.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Alteration of RhoA and ROCKII in GECs of the BTB after overexpression or silencing of miR-200b. (A) Relative mRNA expressions of RhoA and ROCKII as determined by quantitative RT-PCR. GAPDH RNA levels were used as an endogenous control. Values are means ± SD (n = 5/group). ^*P < 0.05 vs. control group; ^#P < 0.05 vs. pre-miR-con group; ^▵P < 0.05 vs. anti-miR-con group;^▿P < 0.05 vs. pre-miR-200b group. (B,C) Protein expressions of RhoA and ROCKII as assessed with Western blot. Values are means ± SD (n = 5/group). ^*P < 0.05 vs. control group; ^#P < 0.05 vs. pre-miR-con group; ^▵P < 0.05 vs. anti-miR-con group;^▿P < 0.05 vs. pre-miR-200b group. (D,E) Distribution and expression of RhoA and ROCKII as detected with immunofluorescence assay. RhoA (green) and ROCKII (green) were, respectively, labeled with fluorescent secondary antibody and nuclei (blue) were labeled with DAPI. Scale bars represent 20 μm.