L-selectin Is Essential to Recruit Protective, Virus-Specific CD8+ T Cells during Primary Vaccinia Virus Infection
(A) Virus titers in ovaries of B6 and RAG1−/− mice at day (d) 5 and day 8 p.i. with vaccNP.
(B and C) Presented are: (B) an experimental plan and (C) virus titers in ovaries of RAG1−/− mice supplemented with naive F5/B6 cells, F5/LΔP CD8+ T cells, or no T cells at day 8 p.i. with vaccNP. One-way ANOVA with post-Tukey’s multiple comparison test.
(D) Bar chart shows MFI of IL-2, TNF-α, IFN-γ, and granzyme B of donor F5/B6 and F5/LΔP CD8+ T cells in ovaries at day 5 p.i. with vaccNP.
(E) Representative density plots show incorporation of EdU dye into the DNA of proliferating donor F5/B6 and F5/LΔP CD8+ T cells in the ovary at day 5 p.i. with vaccNP.
(F) Bar chart shows number of donor F5/LΔP CD8+ T cells in ovaries of RAG1−/− mice treated with MEL-14 or IgG2a antibodies at day 5 p.i. with vaccNP.
Bar charts show means ± SEM. ∗p < 0.05; ∗∗p < 0.005.