Figure 2.

Ordering of Cells through the Developmental Trajectory

(A) We inferred a smooth progression over the developmental lineage, represented as pseudotime, using two different methods. Here we demonstrate the path of both pseudotimes by regressing them into a t-SNE plot of the data. Points are colored based on the cluster the cells belong to.

(B) The pseudotime inferred with two different methods correlate very strongly (Spearman correlation 0.97).

(C) Expression of cd41 mRNA (top), GFP mRNA (middle), and GFP fluorescence (bottom) shown in pseudotime. Each point represents an individual cell; points are colored based on the cluster the cells belong to.

See also Figure S3.