Figure 2.

Lrrc19 KO Mice Are Highly Resistant to DSS-Induced Colitis

(A) Survival (top) and body weight (bottom) were monitored after the start of DSS. WT (n = 18, male) and Lrrc19 KO (n = 18, male) mice were fed a 2% DSS solution in drinking water for 7 days and then switched to regular drinking water.

(B) Length of WT and Lrrc19 KO colon tissues. Mice were sacrificed on day 7 after the start of DSS, and colon length was measured.

(C) H&E staining and histological scores of representative distal colon samples from WT and Lrrc19 KO mice on day 7 after the start of DSS. Histological scores were assessed according to the methods described in the Supplemental Experimental Procedures.

(D) Incidence of colon carcinoma in WT (n = 18, male) and Lrrc19 KO (n = 18, male) mice after AOM-2% DSS treatment for 3 months.

(E) Morphology and tumor numbers of colon carcinoma in WT and Lrrc19 KO mice after AOM-2% DSS treatment for 3 months.

(F) Histopathological changes in representative distal colon samples from Lrrc19 KO and WT mice after staining with H&E.

(G) Immunostaining of PCNA, COX2, and BrdU in colon tissues of representative WT and Lrrc19 KO mice. Colon samples from Lrrc19 KO and WT mice were stained by anti-PCNA or anti-COX2 antibodies. For the BrdU assay, mice were injected intraperitoneally with BrdU, and the colon sections were stained by anti-BrdU antibodies after 4 hr. Brown, PCNA; green, COX2 or BrdU.

Scale bars, 40 μm. ^∗p < 0.05, ^∗∗p < 0.01 (Wilcoxon’s test in A [top], ANOVA in A [bottom], t test in B and D, mean ± SD; Mann-Whitney U test in C and E).