Figure 4.

LRRC19 Deficiency Reduces the Expression of Chemokines CCL6, CCL9, CXCL9, and CXCL10

(A) Microarray analyses of gene expression in WT and Lrrc19 KO mice given standard chow.

(B) qRT-PCR (top) and immunoblot (bottom) of CCL6, CCL9, CXCL9, and CXCL10 in WT and Lrrc19 KO colon epithelial cells.

(C) Size of visible PP and MLN in L19 KO mice with (KO/CC) or without (KO) CCL6-, CCL9-, CXCL9-, and CXCL10-expressing adenovirus injection.

(D) Absolute number of CD4⁺ and CD8⁺ T cells, CD11C⁺MHCII⁺ DCs, F4/80MHCII, and CD11b⁺Gr1⁺ cells in colon tissue of L19 KO mice with or without (control adenovirus only) chemokine adenovirus injection as assessed by flow cytometric analysis. CC, CCL6-, CCL9-, CXCL9-, and CXCL10-expressing adenovirus complexes; WT, cell numbers from the colon tissues of WT mice.

(E) Survival (right) and body weight (left) were monitored until day 14 after the start of DSS. L19 KO mice with (n = 18) or without (n = 18) CCL6, CCL9, CXCL9, and CXCL10 adenovirus injection were fed a 2% DSS solution in drinking water for 7 days and then switched to regular drinking water.

^∗p < 0.05, ^∗∗p < 0.01 (t test in B, mean ± SD; Mann-Whitney U test in C and D; Wilcoxon’s test in E (right); ANOVA in E (left). See also Figure S4.