Figure 3.

Effects of ROC1 knockdown on the mTOR pathway. mTOR pathway-associated protein expression in 253J (A) and EJ (B) cells was examined via western blot. EJ cells were transfected with siRNA for 96 h and then subjected to western blotting analysis (C) and transwell migration assays (D). Representative results of three independent experiments are shown. Columns, means of three independent experiments; bars, s.e.m. *P<0.05. Treated cells were subject to western blot analysis with GAPDH as a loading control, and the quantification relative to GAPDH was conducted by densitometric analysis using Image J software.