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. 2016 Jan 15;6(1):e380. doi: 10.1038/bcj.2015.106

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Copyright © 2016 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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miR-23b promoter is hypermethylated in MM cells. Sequenom's EpiTYPER epigram panel representative of the methylation status (a) of the two CpG islands located within the promoter region of miR-23b or of (b) a 1536 bp region located 843 bp upstream and 597 bp downstream of miR-23b. Methylation data are displayed as color-filled circle and the color spectrum indicates the range of methylation of each CpG. Empty gray circles correspond to CpG sites that failed analysis. Data are reported as mean of three independents experiments. (c) Quantitative reverse transcriptase–polymerase chain reaction analysis for miR-23b expression in KMS11 and MM1S cells treated with 1 μm 5-azacytidine (5-AZA) for 48 h. *P<0.01.

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