FIG 4 .
Expression of KSHV genes in LTC-KMSCs. (A to C) Expression of KSHV latent and lytic genes in LTC-KMSCa (A), LTC-KMSCbm (B), and LTC-KGMSC (C) analyzed by RT-qPCR. Cells infected with KSHV for 4 days were used as controls. β-Actin was used as an internal control. Quantitative values representing the averages ± SDs from three independent experiments are presented. ORF, open reading frame. (D) Expression of KSHV latent and lytic proteins in LTC-KMSCa, LTC-KMSCbm, and LTC-KGMSC analyzed by IFA. iSLK-BAC16 cells induced with doxycycline and sodium butyrate for 2 days were used as positive controls. DAPI, 4′,6-diamidino-2-phenylindole.