FIG 3 .

Axonal IFN pretreatment reduces the number of moving HSV-1 particles, but not LysoTracker-positive organelles in axons. (A and B) IFN-β or IFN-γ was added to the N compartments for 24 h before infection with HSV-1 OK-14. Movement of fluorescent particles in the N compartment was recorded at ~1.5 fps. (A) Stack images of representative movies. Each dot represents a stalled particle, and each line represents a moving particle (bars, 10 µm). (B) Quantification of the percentage of moving particles. (C and D) IFN-β or IFN-γ was added to the N-compartment axons for 24 h before staining with LysoTracker. Movement of fluorescent particles in the N compartment was recorded at ~5 fps. (C) Stack images of representative movies. Each dot represents a stalled particle, and each line represents a moving particle (bars, 10 µm). (D) Quantification of the percentage of moving particles. The results are normalized to the values for no-treatment samples. Data are shown as means plus SEMs. Statistical significance by Student’s t test (n ≥ 3) is indicated as follows: ns, not significant; **, P < 0.005; ****, P < 0.0001.