FIG 6 .

Silencing of JNK, but not PARP, increases cell survival. HeLa cells were treated with siRNAs targeting PARP-1 and JNK and after 72 h infected by a NADase⁺ or NADase⁻ strain, as indicated, and compared to untreated cells (no siRNA) and siControl-treated cells. Following 5 h of infection, the number of cells that were viable was determined by fluorescence microscopy using a vital probe (Live/Dead) as described for Fig. 1. Cells with compromised membrane permeability were considered nonviable. Data are means and SD derived from 3 independent experiments and the examination of at least 1,000 cells. **, P < 0.01; ***, P < 0.001.