FIGURE 2.

Accumulation of defense-related genes and induction of resistance against Phytophthora parasitica in tobacco plants. Effect of wt cryptogein and the mutants on the accumulation of transcripts for PR proteins monitored in the directly infiltrated (A) and surrounding area (B) of leaves or after protein aspiration through petiole of detached leaves (C). Insets represent accumulation of transcripts of wt cryptogein related to control. Gene expression relative to a control was calculated by the ΔΔC(t) method 48 h after proteins application. The values given in the graphs are the log₂R ratio of the relative increase and its standard deviation (SD). A greater than twofold change in transcript accumulation was taken as significant. (D) Eight-week-old tobacco plants were decapitated and treated with elicitins. After 24 h non-necrotic parts of leaves were inoculated with zoospores of P. parasitica. The invaded areas were measured 3 days after inoculation. Each bar represents the standard error of four replicates from three different experiments. Each replicate corresponded to eight inoculated areas on four leaves from one plant. Student’s t-test with p = 0.01 was used to determine whether differences in the area were statistically significant. Different letters denote a significant difference. Cry = wt cryptogein.