FIGURE 8.
Inhibition of recombinant ASIC1a-F350L channels by the sMamb-1 variants F27A, L32A, and L34A. A–C, dose-response curves (% of control current) of the inhibition of F350L mutant ASIC1a channels by sMamb-1 (n = 4) and its variants F27A (A, n = 5), L32A (B, n = 4), and L34A (C, n = 4). Inhibition of ASIC1a by WT sMamb-1 and its variants was described in Fig. 7. D, pharmacological properties of the ASIC1a-F350L inhibition by sMamb-1 and its variants (calculated from data shown in A–C). The fold-change is relative to ASIC1a inhibition by wild-type sMamb-1 (see Table 4). The variation in free energy of interaction (ΔΔGint) was calculated from the IC50 values shown here and in Table 2. For clarity, curves have been split in three panels (A–C) and the same curves for sMamb-1/ASIC1a (see also Fig. 7) and sMamb-1/ASIC1aF350L are shown in all panels.