FIGURE 1.

Npas4 induction is calcium-dependent. MIN6 cells were incubated in basal medium alone (white columns, Basal) or with 40 mm KCl (black columns, KCl) for 2 h before treatment with 5 mm EGTA (a calcium chelator). RNA was extracted and reverse-transcribed, and TaqMan PCR was carried out for Npas4 using GusB as a reference gene and normalizing to control basal (n = 23). Error bars represent mean ± S.E., and significance was determined using a one-way ANOVA with Dunnett post hoc analysis and Bartlett's test for equal variances. ***, p ≤ 0.001.