FIGURE 5.

The MEK1/2, CaMKK, PKC, and CaMKII signaling pathways do not affect Npas4 protein expression. MIN6 cells were kept in either basal medium alone (Basal) or with 40 mm KCl (KCl) for 2 h in the presence of inhibitors or vehicle control. A–H, no decrease in Npas4 protein could be observed using inhibitors of the MEK1/2 (A and B, PD0325901 = 1 μm, n = 4), CaMKK (C and D, STO-609 = 3 μm, n = 4), PKC (E and F, bisindolylmaleimide XI hydrochloride (BIM-XI) = 100 nm, n = 4), or CaMKII (G and H, AIP = 4 μm, n = 4) signaling pathways. The asterisk in A indicates a mobility shift of the NPAS4 band when using the MEK1/2 inhibitor. I and J, unexpectedly, 10 μm compound C caused a decrease in Npas4 protein induction (n = 3). Error bars represent mean ± S.E., and significance was determined using a two-tailed Student's t test with Welch's correction. *, p ≤ 0.05.