Skip to main content
. 2015 Dec 14;291(6):2731–2750. doi: 10.1074/jbc.M115.669614

FIGURE 4.

FIGURE 4.

Generation of XL313 cryptic RGDKGE epitope. A, Western blot of whole cell lysates (left panel) or serum-free conditioned medium (right panel) from stromal cells probed with mAb XL313 or loading control antibodies for β-actin or MMP-9. B, representative examples of FGF-2-stimulated CAM tissues co-stained for expression of avian macrophages (green) and the RGDKGE-containing epitope (red). Scale bars, 50 μm. C, Western blot of whole cell lysates (left panel) or serum-free conditioned medium (right panel) from macrophage cell lines probed with mAb XL313 or loading control antibodies for β-actin or IGFBP-4. D, Western blot of whole cell lysates or serum-free conditioned medium from RAW 264.7 macrophage cells that were either not transfected (WT) or transfected with α2(I) specific shRNA (Col 1 Kd) or control nonspecific shRNA (Cont Kd) with mAb XL313 (left panel) or anti-collagen-1 antibody (right panel).